MiR-503 sensitizes human hepatocellular carcinoma cells to cisplatin by targeting bcl-2

VernacularTitle:MiR-503靶向bcl-2增强BEL-7402细胞对顺铂的敏感性
Author: Xiaoyan YANG ; Jie YIN ; Qiong XIANG ; Hongyan XIE ; Jia YU ; Runliang GAN ; Xiaoyong LEI
Keywords: miR-503; bcl-2; BEL-7402 cells; cisplatin
From: Journal of Central South University(Medical Sciences) 2017;42(6):605-610
CountryChina
Language:Chinese
Abstract: Objective:To investigate effects of miR-503 on cisplatin sensitivity in BEL-7402 cells by targeting of bcl-2.Methods:MiR-503 and bcl-2 mRNA expression levels in hepatocellular carcinoma cells were measured by real-time quantitative (qRT)-PCR;Bcl-protein level was detected by Western blot;miR-503 mimics were transiently transfected to the BEL-7402 cells by liposome transfection;potential target genes of miR-503 were predicted by Bioinformatics software;miR-503 potential targets were validated by dual luciferase activity;and the cell viability was measured by MTT assay.Results:MiR-503 level was down-regulated and Bcl-2 protein expression level was up-regulated in BEL-7402 cells compared with HL-7702 cells.MiR-503 could interact with bcl-2 and inhibit its expression.Cell vitality with miR-503 transfection was significantly reduced compared to that in the negative control.Conclusion:MiR-503 may enhance the sensitivity of BEL-7402 cells to cisplatin and inhibit the cell proliferation by targeting bcl-2.