Role of melatonin in calcium overload-induced heart injury
10.11817/j.issn.1672-7347.2017.06.002
- VernacularTitle:褪黑素在大鼠离体心脏急性钙超载损伤中的作用
- Author:
Lingheng KONG
;
Ming WEI
;
Na SUN
;
Juanxia ZHU
;
Xingli SU
- Keywords:
calcium overload;
melatonin;
cytochrome c;
lactate dehydrogenase;
myocardial injury
- From:
Journal of Central South University(Medical Sciences)
2017;42(6):611-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of melatonin in calcium overload-induced heart injury.Methods:Thirty-two rats were divided into 4 groups:a control group (Control),a melatonin control group (Mel),a calcium overload group (CaP),and a calcium overload plus melatonin group (Mel+CaP).Isolated Sprague Dawley male rat hearts underwent Langendorffperfusion.Left ventricular developed pressure (LVDP) was calculated to evaluate the myocardial performance.Triphenyltetrazolium chloride staining was used to measure the infarct size of myocardium.Lactate dehydrogenase (LDH) activity in the coronary flow was determined.The expressions of caspase-3 and cytochrome c were determined by Western blot.The pathological morphological changes in myocardial fiber were analyzed by HE staining.Results:Compared with the control group,calcium overload significantly induced an enlarged infarct size (P＜0.01),accompanied by the disordered arrangement of myocardial fiber,up-regulation of cytochrome c and caspase-3 (P＜0.01),and the increased activity of LDH (P＜0.01).T hese effects were significantly attenuated by 10 μmol/L melatonin (P＜0.01).Conclusion:Melatonin can alleviate calcium overload-induced heart injury.
