Effects of hypoxia on the growth, mitochondria distribution and function of mouse embryonic fibroblast

VernacularTitle:低氧培养对小鼠胚胎成纤维细胞生长和线粒体分布及功能的影响
Author: Chun WANG ; Hanqing WEI ; Yijin PEI
Keywords: hypoxia; mouse embryonic fibroblasts; mitochondrial distribution; adenosine triphosphate
From: Chongqing Medicine 2017;46(19):2599-2603
CountryChina
Language:Chinese
Abstract: Objective To explore the effects of hypoxia on the growth,mitochondria distribution and function of mouse embryonic fibroblasts(MEFs).Methods MEFs were sub-cultured in the hypoxia group containing 5％ oxygen and normal oxygen group containing 20％ oxygen,every 24 hours,living MEFs were counted by using trypan blue staining.Mito-Tracker Green was used to stain mitochondria,then cells were observed by using laser confocal microscope.The ATP kit was used to detect ATP synthesis.Results During the logarithmic phase,the numbers of living cells in the hypoxia group were higher than those in the normal oxygen group,the differences were statistically significant (P＜0.05).The percentages of perinuclear mitochondrial in the hypoxia group were higher than those in the normal oxygen group,the differences were statistically significant (P＜0.05).Meanwhile,the significant difference was found in the ATP level between the two groups (P＜0.05).Conclusion The distribution of mitochondria in MEFs and energy synthesis are influenced by the hypoxic culture condition,which could be better for promoting cell growth compared with normal oxygen culture condition.