Effect of galectin-3 on ventricular remodeling in rabbits with ischemic cardiac insufficiency
10.3969/j.issn.1000-4718.2017.07.012
- VernacularTitle:Galectin-3在缺血性心功能不全家兔心室重构中的作用
- Author:
Yuehua ZHANG
;
Shuren LI
;
Wenhao DENG
;
Xiao HAO
;
Sha LI
;
Yulong MA
;
Lingling YANG
- Keywords:
Galectin-3;
Heart failure;
Myocardial infarction;
Ventricular remodeling;
Cardiac insufficiency
- From:
Chinese Journal of Pathophysiology
2017;33(7):1226-1230
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationship between galectin-3 (Gal-3) and myocardial fibrosis, and to clarify the role of Gal-3 in ventricular remodeling in rabbits with ischemic cardiac insufficiency.METHODS: A rabbit model of ischemic cardiac insufficiency was established by ligation of the anterior descending branch of the coronary artery.The 20 rabbits were randomly divided into sham operation group and cardiac insufficiency group by random number table method.After 4 weeks of coronary artery ligation, the cardiac function was measured by cardiac echocardiogram.Real-time PCR and Western blot were used to detect the expression of Gal-3, type I collagen and type III collagen at mRNA and protein levels in the myocardium.The serum Gal-3 contents were measured by ELISA.HE staining and Masson staining were used to observe the degree of fibrosis development in myocardial tissues after infarction.RESULTS: Compared with sham operation group, the mRNA expression of Gal-3 in cardiac insufficiency group was significantly increased.At the same time, type I collagen, type III collagen and collagen type I/III ratio were also increased significantly.The protein contents of Gal-3, type I collagen and type III collagen were increased significantly.The serum Gal-3 levels were significantly increased.The pathological changes were observed in cardiac insufficiency group as the myocardial cell morphological disorder and marked hyperplasia of fibrous tissue were seen.CONCLUSION: Gal-3 aggravates myocardial fibrosis in rabbits with ischemic cardiac insufficiency, and promotes the ventricular remodeling and the occurrence of heart failure.
