ILC2 regulates chronic renal failure patients' immunity by secretion of IL-13 through activating of STAT6
10.3969/j.issn.1000-4718.2017.07.024
- VernacularTitle:ILC2通过激活STAT6和分泌IL-13调节慢性肾功能衰竭患者的免疫功能
- Author:
Zhanpeng SUN
;
Xin LI
;
Yan YANG
;
Bo LI
;
Hongyan WEI
;
Chunlin HU
;
Qingling FU
;
Xiaoxing LIAO
- Keywords:
Type 2 innate lymphoid cells;
Flow cytometry;
Chronic renal failure;
Signal transducers and activators of transcription 6;
IL-13
- From:
Chinese Journal of Pathophysiology
2017;33(7):1301-1305
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect and possible mechanism of type 2 innate lymphoid cell (ILC2) on the development of chronic renal failure (CRF).METHODS: The patients with chronic renal failure (n=36) in the Fist Affiliated Hospital of Sun Yat-sen University from March 2016 to December 2016 were selected, and 32 healthy persons in the same period were enrolled in the study for control.The proportion of ILC2 in the PBMC of CRF patients and healthy controls was detected by flow cytometry, IL-13 concentration in the plasma was measured by ELISA.The isolated PBMCs from the patients and healthy persons were divided into 3 groups (control group, cytokine group, intervention group) and cultured in vitro for 3 days, respespestively, then IL-13 concentration was measured by ELISA.The protein levels of phosphorylated signal transducers and activators of transcription 6 (p-STAT6) in the PBMC of healthy controls before stimulation and after stimulation for 15 min, 30 min, 1 h, 2 h were determined by Western blot.RESULTS: The proportion of ILC2 in the PBMC and the plasma IL-13 concentration of CRF patients was higher than that in the healthy controls (P<0.05).In the culture supernatant in vitro, IL-13 concentration in the 3 subgroups of CRF patients (control group, cytokine group, intervention group) were all higher than that in the healthy controls (P<0.05), both the 2 groups showed a trend that the active IL-13 concentration in cytokine group was higher than that in control group, and that in intervention group was lower than that in cytokine group.The protein levels of p-STAT6 in cytokine stimulated-PBMC with a time dependent manner.CONCLUSION: The percentage of ILC2 in the PBMC is elevated in CRF patients.Furthermore, the ILC2 secret large amount of IL-13 to mediate the polarization of Th2 cells to regulate immunity through activating p-STAT6.
