Effect of miR-200c retardation transforming growth factor-β1 in retroperitoneal fibroblast proliferation and migration
10.3969/j.issn.1000-484X.2017.07.010
- VernacularTitle:miR-200c阻滞转化生长因子-β1在腹膜后成纤维细胞增殖和迁移侵袭中的作用
- Author:
Ying SHI
;
Ling LIN
;
Jiayin KAN
;
Haiyan LIU
;
Zengwei MA
;
Shuangyu LI
- Keywords:
miR-200c;
Fibroblasts;
Proliferation;
Migration
- From:
Chinese Journal of Immunology
2017;33(7):1005-1008
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clarify the effect of miR-200c on the proliferation of retroperitoneal fibroblast, and analyze its molecular mechanism, which provide theoretical basis for the inhibition of retroperitoneal fibrosis. Methods: 36 cases of retroperitoneal tissues were collected, and the primary fibroblasts were cultured. The cells stimulated by 5 ng/ml transforming growth factor-β1 (TGF-β1) were used as TGF-β1 group;the cells were transfected with pcDNA3.1-miR-200c first, and then stimulated by 5 ng/ml TGF-β1 were used as miR-200c group;the cells transfected only by pcDNA 3.1 were used as pcDNA 3.1 group;the normal cultured cells were used as control group. The MTT assay, cell scratch and Transwell chamber were used to detect the proliferation and migration of fibroblast, respectively. The ELISA experiment was used to detect the content of Akt protein in the lysis liquid of each group. Results: The OD value of fibroblast proliferation in miR-200c group was obviously lower than that of TGF-β1 group (P<0.01);compared with TGF-β1 group, the cell migration rate was decreased significantly in miR-200c group (P<0.01);the content of Akt protein in miR-200c was obviously lower than that of TGF-β1 group (P<0.01). Conclusion: miR-200c inhibits the effect of TGF-β1 on the proliferation and migration of fibroblast by down-regulating Akt signaling pathway, which has important significance in inhibiting peritoneal fibrosis.
