Effects of total flavonoids from Cycas Revolute on expression of VEGF,bFGF,HIF-1α and NF-κB in model mice of Lewis lung cancer
10.3969/j.issn.1000-484X.2017.07.015
- VernacularTitle:苏铁总黄酮对Lewis肺癌模型小鼠VEGF、bFGF、HIF-1α、NF-κB表达的影响
- Author:
Shaohui WANG
;
Sibu MA
;
Yu YAN
;
Zhekang CHENG
;
Yachen ZHANG
;
Tongxiang LIU
- Keywords:
Total flavonoids from Cycas Revolute;
Lewis lung cancer;
VEGF;
bFGF;
HIF-1α;
NF-κB
- From:
Chinese Journal of Immunology
2017;33(7):1029-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of total flavonoids from Cycas Revolute on expression of vascular endothelial growth factor (VEGF),basic fibroblast growth factor (bFGF),Hypoxia inducible factor-1α (HIF-1α) and nuclear factor-κB(NF-κB) in model mice of Lewis lung cancer.Methods: The expressions of VEGF,bFGF,HIF-1α and NF-κB in tumor tissues were detected by immunohistochemistry and Western blot.The expression of VEGF,VEGF and NF-κB in tumor tissues were detected by fluorescent quantitative PCR.BFGF,HIF-1α and NF-κB mRNA were detected by immunohistochemistry.Results: The results of immunohis-tochemistry,Western blot and Real-time PCR showed that the results were basically the same,compared with model group,the expression of VEGF,bFGF,HIF-1α,NF-κB mRNA and the expression of VEGF,bFGF,HIF-1α and NF-κB were decreased,the difference was highly significant (P<0.05).Conclusion: The mechanism of total flavonoids from Cycas Revolute in the treatment of lung cancer may be through inhibition of the expression of VEGF,bFGF,HIF-1α,NF-κB in invasion and metastasis,and further inhibit the expression of VEGF,bFGF,HIF-1α and NF-κB in invasion and metastasis-related proteins,thus play a role of anti-lung cancer invasion and metastasis.
