Degranulation and anti-inflammatory effects of shikimic acid in Chaenomeles speciosa on peritoneal mast cells in rats
10.3969/j.issn.1000-484X.2017.07.016
- VernacularTitle:资木瓜中莽草酸对大鼠腹腔肥大细胞脱颗粒及抗炎作用研究
- Author:
Qianqian ZHENG
;
Tiantian DONG
;
Shigang LI
;
Wei LIU
;
Lingling YU
;
Xianyong CHEN
;
Jinling WANG
- Keywords:
Chaenomeles speciosa;
Shikimic acid;
RPMC;
β-hexos1 minidase;
Histamine
- From:
Chinese Journal of Immunology
2017;33(7):1035-1038
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Chaenomeles speciosa total extract shikimic acid(SA) on the degranulation of compound 48/80(C48/80) stimulated rat peritoneal mast cells and the anti-inflammatory.Methods: Qualitative analysis of shikimic acid in the total extract of Chaenomeles speciosa by high performance liquid phase;RPMC were identified by toluidine blue,immunohistochemistry and electron microscopy;CCK-8 method was used to detect the proliferation of RPMC in each concentration group.The release rate of β-hexosidase was determined by substrate method.The content of histamine in supernatant was detected by ELISA.Results: SA had no significant effect on the growth of RPMC at the concentration of 0-90 μg/ml.Compared with the positive control group,SA could effectively inhibit the release of β-hexosidase and inhibit the secretion of histamine.Conclusion: SA inhibits the release of histamine in inflammatory mediators by inhibiting the degranulation of RPMC stimulated by C48/80,and then exerts anti-inflammatory effects.
