Research of RBP1 like protein--a novel autoantigen in RA

VernacularTitle:类风湿性关节炎新型自身抗原视黄醇结合蛋白1类似蛋白相关分析研究
Author: Xiaopeng TONG ; Min ZHANG
Keywords: RA; Autoantigen; RBP1; Cloning; Immunology
From: Chinese Journal of Immunology 2017;33(7):976-978
CountryChina
Language:Chinese
Abstract: Objective:Rheumatoid arthritis(RA) is a systemic autoimmune disease,but its etiology is unclear.Therefore,it is urgent to search antigens which is relate to induce this disease,however,there are few reports about the new self-antigens at home and abroad.In this study,we investigate new autoantigen through cloning.Methods: We used antigens in synovium of patients with rheumatoid arthritis as probe,extracted mRNA from synoviocyte,performed cDNA library immunological screening and sequence,and analyzed the conformation and performed Northern blotting.Results: We found that new autoantigen had 36.5% homology with retinoblastoma binding protein 1(RBP1)and it was found that these special protein structure such as AT-rich interaction domain,chromo domain,A+T-hook and TAF homology domain.Therefore,it will be named RBP1-like Protein(Rbik).Rbik RNA is not only expressed in the synovial membrane,but also in the heart,small intestine,muscle and other organs.Conclusion: We report that the discovery of the new autoantibodies to RBP1-like Protein(Rbik)may provide a new possible index and target in diagnosis and therapy.