Expression and significance inflammatory of cytokine mediated by Gli1 in acute pancreatitis
10.3969/j.issn.1000-484X.2017.07.021
- VernacularTitle:急性胰腺炎中Gli1介导的炎性细胞因子的表达变化及意义
- Author:
Kun LAI
;
Xuemei HE
;
Lan LUO
;
Fang WANG
;
Xiangyu ZHOU
- Keywords:
Gli1;
Remote tissue;
Acute pancreatitis;
Inflammatory cytokine
- From:
Chinese Journal of Immunology
2017;33(7):1057-1061
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and the significance of inflammatory cytokine IL-6 through regulating Gli1 in acute pancreatitis.Methods: In this study,C57 mice were randomly divided into three groups:control group,model group,inhibitor group.caerulein intraperitoneal injection induce acute pancreatitis model.Use HE staining and amylase to testify the model successfully.Use RT-qPCR,Western blot to detect the expression of Gli1 in the pancreas,liver,lung,kidney and intestine and ELISA method to detect inflammatory cytokines IL-6.Results: Compared with control group,the expression of Gli1 is higher in model group,then the expression of IL-6 increases in inhibitor group which uses Gant61 to suppress Gli1 compared with model group.Conclusion: Gli1 may involved in the process of the distant tissue injury and repair in acute pancreatitis and through regulate its downstream cytokines like IL-6 to play a protective role in acute pancreatitis.
