Expressions of interleukin-11 and interleukin-11 receptorαin non-small cell lung cancer cell lines and their clinical significances
10.3760/cma.j.issn.1006-9801.2017.07.005
- VernacularTitle:白细胞介素11及白细胞介素11受体α在非小细胞肺癌细胞株中的表达及其意义
- Author:
Yi CHANG
;
Mu HU
;
Xiuyi ZHI
;
Xiuhong NIE
- Keywords:
Carcinoma,non-small-cell lung;
Interleukin-11;
Interleukin-11 receptor alpha subunit;
Diagnosis
- From:
Cancer Research and Clinic
2017;29(7):453-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expressions of interleukin-11 (IL-11) and interleukin-11 receptorα(IL-11Rα) in non-small cell lung cancer (NSCLC) cell lines, and explore their clinical significances. Methods The expressions of IL-11 and IL-11Rαin NSCLC cell lines A549, H2228, healthy lung small airway epithelial cell (SAEC) line cytoplasm, cell membrane and nucleus were detected by Western blot. Results The expressions of IL-11 and IL-11Rα were low in the cell membrance and nucleus (cell membrane: IL-110.04± 0.03, IL-11Rα0.05±0.03; nuclear: IL-110.45±0.19, IL-11Rα0.07±0.02;P<0.01); The expressions of IL-11 and IL-11Rα in A549 and H2228 cell lines were significantly increased compared with those of SAEC cell lines in the cell membrance and cytoplasm (P< 0.01); Among the A549 cell lines, the expressions of IL-11 and IL-11Rα in cell nucleus were much higher than those of the cell membrance and cytoplasm (P< 0.01). Among the H2228 cell lines, the expression of IL-11 in cytoplasm was the highest and the expression of IL-11Rα was the highest in the cell nucleus (P< 0.01). Conclusion The expressions of IL-11 and IL-11Rαare high in NSCLC cell lines, and it is good for the screening and early diagnosis of lung cancer by detecting the expressions of IL-11 and IL-11Rα.
