Role of nucleolin on myocardial hypertrophy and fibrosis in type II diabetic cardiomyopathy mice
10.3969/j.issn.1000-4718.2017.07.013
- VernacularTitle:核仁素对小鼠糖尿病性心肌病心肌肥大与纤维化发生的影响
- Author:
Qinglan Lü
;
Li SUN
;
Bimei JIANG
;
Yanjuan LIU
;
Yuanbin LI
;
Guangwen CHEN
;
Meidong LIU
;
Xianzhong XIAO
- Keywords:
Diabetic cardiomyopathy;
Nucleolin;
Myocardial hypertrophy;
Fibrosis;
Transgenic mice
- From:
Chinese Journal of Pathophysiology
2017;33(7):1231-1236
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of nucleolin on diabetic cardiomyopathy in mice.METHODS: A type II diabetic cardiomyopathy mouse model was prepared using a cardiac-specific nucleolin-overexpressing transgenic mice.The mice were divided into wild-type mouse control group, nucleolin transgenic mouse control group, wild-type mouse diabetes group and nucleolin transgenic mouse diabetes group.Wheat germ agglutinin (WGA) fluorescent dye, Masson staining and PowerLab system detection were used to further clarify the role of nucleolin on cardiac hypertrophy, fibrosis and cardiac function in type II diabetic cardiomyopathy mice.RESULTS: Compared with wild-type mouse control group, no significant increase in blood glucose level was found, while genetical myocardial cell hypertrophy was significantly attenuated in nucleolin transgenic mouse diabetes group.The collagen fibers were also significantly reduced, and hemodynamic indexes ± dp/dtmax, left ventricular end-diastolic pressure, left ventricular systolic pressure and heart rate were also improved.The above differences were statistically significant.CONCLUSION: Nucleolin may reduce the occurrence of myocardial hypertrophy and fibrosis, thus improving the cardiac function of diabetic cardiomyopathy mice.
