Astragalus membranaceus inhibits bleomycin-induced pulmonary fibrosis in mice by antioxidation
10.3969/j.issn.1000-4718.2017.07.019
- VernacularTitle:黄芪通过抗氧化抑制博莱霉素诱导的小鼠肺纤维化
- Author:
Ouyang YAN
- Keywords:
Astragalus membranaceus;
Pulmonary fibrosis;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2017;33(7):1271-1277
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of Astragalus membranaceus on the balance of oxidation and antioxidation in bleomycin-induced pulmonary fibrosis in mice and the possible anti-fibrosis mechanism of Astragalus membranaceus.METHODS: Female KM mice (n=36) were randomly divided into 3 groups.The mice in control group were administered with saline aerosol intratracheally.The mice in fibrosis group were administered with bleomycin at dose of 3 mg/kg aerosol intratracheally.The mice in Astragalus membranaceus group were administered with bleomycin at dose of 3 mg/kg aerosol intratracheally and then intraperitoneal injected with Astragalus membranaceus parenteral solution at daily dose of 1.7 g/kg.All mice were sacrificed 14 d after the treatment, and the lungs and serum were collected for detection.Hematoxylin-eosin staining was performed in the lung tissue.The mRNA expression of superoxide dismutase 1/2/3 (SOD1/2/3), catalase (CAT), NADPH oxidase 2/4 (NOX2/4) and α-smooth muscle actin (α-SMA) was detected by RT-PCR, and the protein expression of α-SMA and NOX2/4 was determined by Western blot.The concentration of malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in the serum were measured by a colorimetric method.RESULTS: The pathological injury was obviously observed in bleomycin group compared with control group, but was attenuated in Astragalus membranaceus group.The α-SMA mRNA and protein expression, MDA/T-AOC, NOX2, NOX4 and SOD3 mRNA expression, and NOX2 protein expression in bleomycin group were significantly higher than those in control group, while those in Astragalus membranaceus group were significantly lower than those in bleomycin group.The protein expression of NOX4 in bleomycin group was significantly lower than that in control group, while that in Astragalus membranaceus group was higher than that in bleomycin group.The mRNA expression of SOD1 and CAT in Astragalus membranaceus group and bleomycin group were decreased compared with control group.No significant difference of SOD2 mRNA expression among the 3 groups was observed.CONCLUSION: Astragalus membranaceus inhibits bleomycin-induced pulmonary fibrosis in mice by maintaining the balance of oxidation and antioxidation.
