Effects of Rab7 gene silencing on cytokine and MAPK signal pathway activated by R848 in macrophage

VernacularTitle:Rab7基因沉默对巨噬细胞中R848激活的细胞因子及MAPK信号通路影响
Author: Kai ZOU ; Xiaole YUN ; Hongbin KANG ; Xue WANG ; Xiaohui ZHANG ; Jiming XIE ; Yuzhen WANG
Keywords: Rab7; TLR7; Raw264.7 cell; MAPK
From: Chinese Journal of Immunology 2017;33(7):967-970
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of Rab7 on cytokine induced by TLR7 (Toll like receptor-7) R848 activated in Raw264.7,and discusses the influence of Rab7 on MAPK signal transduction.Methods: TLR7 downstream cytokines such as TNF-α,IL-6,IFN-α,IFN-β and IP-10 activated by R848 were detected through Q-PCR in Rab7 silenced mouse macrophages,and then analysis of phosphorylation of MAPK determined with Western blot showed the effect of Rab7 on signal transduction of MAPK.Results: Rab7 inhibit production of cytokine activated by TLR7,and also,Rab7 had an inhibitory effect on MAPK signal pathway.Conclusion: The experimental results further illustrate that the Rab7 is the TLR7 signal transduction pathway negative regulatory factor,and to participate in MAPK signaling pathway.