Impact and mechanism of leptin on bone marrow-derived dendritic cells(BMDCs)via NLRP3 inflammasome
10.3969/j.issn.1000-484X.2017.07.003
- VernacularTitle:瘦素通过NLRP3炎性体对小鼠骨髓树突状细胞的作用及机制
- Author:
Yiyun YU
;
Sisi FU
;
Lei LIU
;
Jie YANG
;
Lei HAN
;
Ling Lü
- Keywords:
Leptin;
NLRP3 inflammasome;
Dendritic cells;
Cytokine
- From:
Chinese Journal of Immunology
2017;33(7):971-975
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and mechanism of leptin on dendritic cells by NLRP3 inflammasome.Methods: BMDCs were induced in vitro,leptin with scalar doses was cocultured with BMDCs,IL-1β and IL-18 mRNA expression and protein secretion level were measured by q-RT-PCR and ELISA respectively.Caspase-1 activity or ROS synthesis were tested with FLICA kit or ROS detection assay kit on flow cytometry.IL-1β or IL-18 were detected after caspase-1 was inhibited by Ac-YVAD-cmk or NLRP3 was interfered by siRNA or ROS inhibitor DPI or KCL were added.Results: Leptin promoted secretion of IL-1β and IL-18.Leptin up-regulated NLRP3 and activted caspase-1 to secret proinflammtory cytokine,which K+ efflux took part in.Conclusion: Leptin promotes secretion of IL-1β and IL-18 by activating NLRP3 inflammasome,and K+ efflux takes part in this,which hints us that leptin may be an activator of NLRP3 inflammasome.
