Role of CD46 and Nectin-4 on Measles virus (MV) infecting human pulmonary alveolar epithelial cells
10.3969/j.issn.1000-484X.2017.07.007
- VernacularTitle:CD46、Nectin-4在麻疹病毒感染人肺泡上皮细胞中的作用
- Author:
Tianjing CAI
;
Zhiliang LI
;
Lingying JIN
;
Yunbo GAN
- Keywords:
Measles virus;
Human pulmonary alveolar epithelial cells;
CD46;
Nectin-4
- From:
Chinese Journal of Immunology
2017;33(7):991-994
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of CD46 and Nectin-4 on Measles virus (MV) infecting human pulmonary alveolar epithelial cells (HPAEpiC),and the interaction between CD46 and Nectin-4.Methods: Measles virus was divided into pre-infection group and 2 h-infection group,HPAEpiCs treatment with anti-CD46 antibody and/or anti-Nectin-4 antibody as experimental groups,and untreated HPAEpiCs as a control.The variation of viral replication level was detected.A Co-immunoprecipitation assay (Co-IP) was used to explore whether CD46 and Nectin-4 had interactive relationship in MV infection.Results: Compared with the control group,MV titers were reduced in HPAEpiCs of the pre-infection group treated with anti-CD46 and anti-Nectin-4 respectively (48.03% and 49.53%).Furthermore,virus titers showed a more reduction in which treated with anti-CD46 and anti-Nectin-4 antibodies (27.15%,P<0.01).Western blot and Real-time PCR showed that anti-CD46 antibody and anti-Nectin-4 antibodies decreased the rate of MV infection.In the 2 h-infection group,however,the treatment with anti-CD46 and anti-Nectin-4 could significantly reduce the MV titer and NP protein in HPAEpiCs.The Co-IP assay showed that there were interaction between CD46 and Nectin-4.Conclusion: CD46 and Nectin-4 mediated MV infecting HPAEpiCs.Moreover,CD46 and Nectin-4 may play a synergetic role in MV infection,which could enhance the infection effect.
