Recombinant IFN-α-IL-18 increases level of histamine produced by chicken lymphocyte and activate NF-κB p65 activity
10.3969/j.issn.1000-484X.2017.07.008
- VernacularTitle:重组IFN-α-IL-18对鸡淋巴细胞组胺的诱导生成和NF-κB p65的激活
- Author:
Danfang YANG
;
Yinju LI
;
Hui YANG
;
Yichen LIU
;
Xiangchao CHENG
;
Chunjie ZHANG
- Keywords:
Interferon-α-interleukin-18;
Histidine decarboxylase;
Histamine;
PI3K;
NF-κB
- From:
Chinese Journal of Immunology
2017;33(7):995-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the effects of recombinant IFN-α-IL-18 fusion protein on chicken lymphocyte histamine induced and the NF-κB p65 activation and nucleo-cytoplasmic transport.Methods: The healthy chickens blood was sterile adopted with anticoagulant,then separation of the chicken peripheral blood lymphocyte and divided into 10 groups:The yeast expression and purification protein IFN-α-IL-18,IL-18,IFN-α were added with 250 ng/ml,500 ng/ml and 1 000 ng/ml respectively while the control was only added RPMI1640 with 3 repetitions for each group.Then the histidine decarboxylase activity,histamine,IFN-γ,PI3K,MAPK and NF-κB p65 in cell nucleus were detected.Results: The recombinant IFN-α-IL-18 and IL-18 could significantly promote the activity of histidine decarboxylase (P<0.01),increase the contents of histamine (P<0.01),induce IFN-γ (P<0.01),improve the contents of PI3K (P<0.01) and the NF-κB p65 levels in nucleus (P<0.01),and the higher concentration of IFN-α had a similar effect to lymphocytes.The effects of IFN-α-IL-18,IL-18 and IFN-α on MAPK was acratia.Conclusion: IFN-α-IL-18 and IL-18 can stimulate chicken peripheral blood lymphocyte populations increased histamine contents significantly and promote the induction of IFN-γ.IFN-α-IL-18,IL-18 and IFN-α increase PI3K expression in lymphocyte associated with the NF-κB activation and NF-κB p65 nucleo-cytoplasmic transport.The study built foundation for the function of IFN-α-IL-18 and the exploration of the mechanism of controlling epidemic diseases.
