Nutlin-3 promotes pyroptosis in SMMC-7721 cells
10.3969/j.issn.1000-484X.2017.07.012
- VernacularTitle:Nutlin-3促进肝癌细胞SMMC-7721发生焦亡的作用
- Author:
Xinli SHI
;
Jingli LIU
;
Kun YU
;
Jie LIANG
;
Xiaoli NIU
- Keywords:
Nutlin-3;
Hepatocellular carcinoma;
Pyroptosis;
caspase-1;
IL-1β
- From:
Chinese Journal of Immunology
2017;33(7):1014-1017
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of Nutlin-3,a MDM2 antagonist,on the pyroptosis in SMMC-7721 cells.Methods: The expression of actived caspase-1(p20) and IL-1β was detected using Western blot analysis.Pyroptosis was investigated by a standard lactate dehydrogenase release assay(LDH).IL-1β content in the cell culture supernatant was quantified by ELISA.Results: Nutlin-3 up-regulated the expression level of actived caspase-1 and IL-1β in SMMC-7721 cells.Meanwhile,Nutlin-3 increased significantly the content of LDH and IL-1β in the cell culture supernatant(P<0.05).Conclusion: Nutlin-3 activated pro-caspase-1,promoted pyroptosis and IL-1β release in SMMC-7721 cells.
