Highuric acid induced insulin resistance in 3T3-L1 adipocyte and its mechanism
10.3969/j.issn.1006-6187.2017.07.015
- VernacularTitle:高尿酸诱导3T3-L1脂肪细胞胰岛素抵抗及其机制的研究
- Author:
Wanling XU
;
Hui LIU
;
Lei YUAN
- Keywords:
High uric acid;
Insulin resistance;
3T3-L1 adipocytes;
Insulin receptor substrate;
Glucose transporter 4
- From:
Chinese Journal of Diabetes
2017;25(7):644-648
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of high uric acid(HUA) on insulin sensitivity (IS) in 3T3-L1 adipocytes and its mechanism. Methods 3T3-L1 adipocytes were pretreated with HUA with or without NAC,and then stimulated by insulin. The cell viability of 3T3-L1 adipocytes was detected by CCK-8 assay. The glucose consumption was measured by glucose oxidase method. The levels of phospho-IRS-1,phospho-Akt and GluT4 protein were tested by western blot. Results HUA could inhibit insulin-induced glucose consumption,phosphorylation of Akt (Thr308),dephosphorylation of IRS-1 (Ser307) and the GluT4 protein expression (P<0.05). All the above could be blocked by NAC(P<0.05). Conclusion HUA can inhibit inulin stimulated IRS-1/Akt signaling and GluT4 expression,and thus induced insulin resistance in 3T3-L1 adipocytes.
