Effects of Biejiajian Pills on NF-κB signaling pathway in hepatic stellate cells in rats
10.3969/j.issn.1001-1528.2017.07.006
- VernacularTitle:鳖甲煎丸对大鼠肝星状细胞中NF-κB信号通路的影响
- Author:
Guanxin CHEN
;
Bin WEN
;
Haitao SUN
;
Jialing SUN
;
Wei XU
;
Haiyan AN
;
Songqi HE
- Keywords:
Biejiajian Pills;
hepatic stellate cells;
NF-κB;
IκB;
α-SMA;
VEGF;
TIMP-1
- From:
Chinese Traditional Patent Medicine
2017;39(7):1355-1360
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the effects of Biejiajian Pills (Colla Carapacis Trionycis,Asini Corii Colla,Nidus Vespae,etc.) on NF-κB,p65,p50 and IκB in NF-κB signaling pathway and target gene expression in HSC-T6 cells of rats.METHODS HSC-T6 cells were cultured with Biejiajian Pills drug serum for 24 hours,the expressions of p65,p50,VEGF and TIMP-1 mRNA were determined by qPCR;the expression of p65 was measured by immunofluorescence;the expressions of IκBα,IκBβ and α-SMA were determined by Western blot.RESULTS The Biejiajian Pills middle-,high-dose and positive control groups showed significantly lower expressions of p65,VEGF and TIMP-1 mRNA as compared with the blank control group and negative control group,the expressions of p50 mRNA among various groups showed no significant differences.But immunofluorescence showed that the expression of p65 in cytoplasm was decreased.Meanwhile,Biejiajian Pills showed significantly higher IκBα protein expression and obvious down-regulation of α-SMA expression in a dose-dependent manner,but had no significant influence on the expression of IκBβ.CONCLUSION Biejiajian Pills' therapeutic effects on hepatic fibrosis may be related to influencing NF-κB signaling pathway and inhibiting the expression of down-stream target gene.
