Simultaneous determination of ten constituents in Zhenqi Fuzheng Capsules by UPLC-MS/MS
10.3969/j.issn.1001-1528.2017.07.015
- VernacularTitle:UPLC-MS/MS法同时测定贞芪扶正胶囊中10种成分
- Author:
Guizhen FANG
;
Xiao LIU
;
Yunyun SHEN
;
Haiying MO
;
Baoxia LIU
;
Ruihong LI
;
Jinlong QI
- Keywords:
Zhenqi Fuzheng Capsules;
chemical constituents;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2017;39(7):1398-1402
- CountryChina
- Language:Chinese
-
Abstract:
AIM To develop a UPLC-MS/MS method for the simultaneous content determination of adenosine,salidroside,chlorogenic acid,calycosin-7-O-β-D-glycoside,specnuezhenide,calycosin,formononetin,quercetin,apigenin and astragaloside Ⅳ in Zhenqi Fuzheng Capsules (Ligustri lucidi Fructus and Astragali Radix).METHODS The analysis of methanol extract of this drug was performed on a 40 ℃ thermostatic Inertsutain C18 column (75 mm × 3.0 mm,2 μm),with the mobile phase comprising of methanol-acetonitrile-4 mmol/L ammonium acetate flowing at 0.5 mL/min in a gradient elution manner.RESULTS Ten constituents showed good linear relationships within their own ranges (r≥0.996 0),whose average recoveries were 95.1％-104.3％ with the RSDs of less than 4.20％.CONCLUSION This sensitive and specific method can be used for the rapid quality control of Zhenqi Fuzheng Capsules.
