Simultaneous determination of eight constituents in Jinsang Liyan Capsules by HPLC
10.3969/j.issn.1001-1528.2017.07.016
- VernacularTitle:HPLC法同时测定金嗓利咽胶囊中8种成分
- Author:
Jilong CHEN
- Keywords:
Jinsang Liyan Capsules;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2017;39(7):1402-1405
- CountryChina
- Language:Chinese
-
Abstract:
AIM To develop an HPLC method for the simultaneous content determination of honokiol,magnolol,dehydrotumulosic acid,tumulosic acid,polyporenic acid C,3-epidehydrotumulosic acid,dehydropachymic acid and pachymic acid in Jinsang Liyan Capsules (Magnoliae officinalis Cortex,Poria,Aurantii Fructus Immaturus,etc.).METHODS The analysis of methanol extract of this drug was carried out on a 35 ℃ thermostatic Kromasil C18 column (4.6 mm × 250 mm,5.0 μm),with the mobile phase comprising of acetonitrile-0.05% phosphoric acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 294,241 and 210 nm.RESULTS Eight constituents showed good linear relationships within their own ranges (r > 0.999 0),whose average recoveries were more than 96.0% with the RSDs of less than 2.0%.CONCLUSION This simple and reproducible method can be used for the quality control of Jinsang Liyan Capsules.