Accurate determination of HLA ambiguous results based on group-specific haploid full-length sequencing

VernacularTitle:基于HLA组特异性单倍体全长测序方法对模棱两可结果的准确定型
Author: Songxing WANG ; Hui YANG ; Liumei HE ; Wenxu HONG ; Hongyan ZOU ; Yunping XU
From: Chinese Journal of Tissue Engineering Research 2017;21(20):3208-3215
CountryChina
Language:Chinese
Abstract: BACKGROUND: Due to the polymorphism of HLA, a large number of ambiguities have been generated by conventional HLA typing techniques, and confirmed stereotypes of ambiguous results based on group-specific haploid full-length typing are rarely reported.OBJECTIVE: To analyze the accuracy of HLA-typing ambigulity based on group-specific haploid full-length sequencing. METHODS: The low-resolution results were used as the starting point for two ambiguous samples. Sanger sequencing (PCR-SBT) based on haploid full-length was performed after group-specific amplification. RESULTS AND CONCLUSION: One case showed a new A*02:03:01 allele, which was found a mutation in NT817 from C to T in comparison with A*11:01:01:01. The other case indicated another new C*07:02:01:01, which was found a mutation in NT879 from A to G in comparison with C*08:01:01. In conclusion, these results indicate that the group-specific haploid full-length sequencing method can be used to accurately classify HLA alleles and to discover new alleles.