Noise-Induced Acetyl-histone H2B Expression Alteration in Cochlear Basilar Membrane of Guinea Pigs
10.3969/j.issn.1006-7299.2017.04.016
- VernacularTitle:噪声对豚鼠耳蜗基底膜乙酰化组蛋白H2B表达的影响
- Author:
Denghua YANG
;
Shusheng GONG
;
Liyan CHEN
;
Jing XIE
- Keywords:
Noise-induced hearing loss;
Histone;
Histone acetyltransferase;
Histone deacetylase;
Hair cell
- From:
Journal of Audiology and Speech Pathology
2017;25(4):401-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of acetyl-histone H2B in the cochlear hair cells of the guinea pig model of noise-induced hearing loss (NIHL).Methods Sixty guinea pigs were randomly divided into control and noise-exposure group.Thirty guinea pigs in the noise-exposure group were exposed to narrowband noise at 122 dB SPL for 3 hours , while thirty guinea pigs in the control group were not exposed to noise.Auditory thresholds were assessed by auditory brainstem response (ABR), prior to noise in two groups and 3, 7,14 and 21 days after noise exposure in the noise group.Then we investigated the expression of acetyl-histone H2B levels in the sensory cells of basilar membrane after noise expose by immunofluorescence staining and western blot.Results Compared with pre-exposure hearing, ABR thresholds were increased at 1h, 3, 7, 14 and 21 days after noise exposure, and recovered gradually with time and reached a permanent threshold shift at 14 days.The expression of acetyl-histone H2B was down-regulated in the hair cells and Hensen''s cells of the guinea pig cochlea after the noise expose , The ratio of H2B-AcK5 / β-actin was 0.6179±0.1260 in the control group and 0.3102±0.0839 in the noise group, and the difference was statistically significant (P<0.01).Conclusion Noise decreased the inner ear sensory cells histone acetylation level and histone acetylation imbalance may be involved in the occurrence of NIHL.
