Application of immunoglobulin gene rearrangement-derived real-time quantitative polymerase chain reaction in monitoring minimal residual disease of B-cell lymphoblastic leukemia
10.3760/cma.j.issn.1009-9921.2017.07.002
- VernacularTitle:依据免疫球蛋白基因重排构建的实时定量聚合酶链反应体系在B淋巴细胞白血病微小残留病监测中的应用
- Author:
Dali CAI
;
Linlin GAO
;
Qi BI
;
Nan SU
;
Di DAI
;
Shitong CHENG
;
Yan LI
;
Xiaolin GUO
- Keywords:
Leukemia;
B-cell;
Neoplasm;
residual;
Immunoglobulin gene rearrangement;
Polymerase chain reaction
- From:
Journal of Leukemia & Lymphoma
2017;26(7):390-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a real-time quantitative polymerase chain reaction (qPCR) assay for B-cell lymphoblastic leukemia according to individualized and specific immunoglobulin gene rearrangements in leukemia cells, and to use it for the monitoring of minimal residual disease (MRD) of B-cell lymphocytic leukemia. Methods The immunoglobulin gene rearrangements of bone marrow samples from 15 cases of B-cell lymphoblastic leukemia were analyzed with a validated European BIOMED-2 system, and the individualized and specific qPCR-based quantification of leukemic immunoglobulin gene rearrangements was established. Results Unique and specific gene rearrangements of immunoglobulin light and heavy chains were identified in 14 cases and Ig-qPCR based on these gene rearrangements had a sensitivity of 10-5 and high specificity which met the international criteria in 10 patients. Leukemia MRD quantification with immunoglobulin gene rearrangement-based qPCR was similar as compared with other MRD detection methods. Conclusion Immunoglobulin gene rearrangement-based leukemia MRD quantification is feasible, sensitive, specific, precise and much valuable for clinical decision of treatments in B-cell lymphoblastic leukemia.
