Study on the Mechanism of Ulinastain Inhibiting Inflammatory Reaction of Patients Underwent Cardiopul-monary Bypass Cardiac Surgery by Inducing miR-155
10.6039/j.issn.1001-0408.2017.20.02
- VernacularTitle:乌司他丁通过诱导miR-155抑制体外循环心脏手术患者炎症反应的作用机制研究
- Author:
Liqing XU
;
Jiangqi WEI
- Keywords:
Ulinastain;
Cardiopulmonary bypass;
Cardiac surgery;
miR-155;
Inflammatory reaction
- From:
China Pharmacy
2017;28(20):2742-2745
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the mechianism of inhibitory effect of ulinastain on inflammatory factors of patients during cardiopulmonary bypass(CPB)cardiac surgery. METHODS:Totally 40 patients underwent selective CPB cardiac surgery collected from our hospital during Jul. 2012-Jul. 2016 were divided into control group and observation group according to random number ta-ble,with 20 cases in each group. Observation group was given 300000 U of ulinastatin at 2.5 mL/min by intravenous pump within 20 min after anesthesia induction, then given 700000 U of ulinastatin at 0.2 mL/min by continuous intravenous pump until opera-tion finish. Control group was given equal volume of normal saline. Blood samples of patients were collected before anesthesia in-duction (before surgery) and 6,12,24 h after CPB (after surgery),respectively. The expression of miR-155 and its target gene MyD88 in peripheral blood mononuclear cells of each group were detected by Real-time PCR and Western blot. The expression of TNF-α,IL-6 and IL-8 were measured by ELISA. The correlation of miR-155 with the expression of TNF-α,IL-6 and IL-8 in pa-tients of observation group 24 h after surgery were analyzed by using Pearson correlation analysis. RESULTS:Compared with con-trol group,the expression of miR-155 in observation group was significantly increased,while the expression of its target gene MyD88 was significantly decreased;the expression of TNF-α,IL-6 and IL-8 were reduced significantly,with statistical signifi-cance(P<0.01). The expression of miR-155 was significantly negative correlation with the expression of TNF-α,IL-6 and IL-8 in the patients of observation group 24 h after surgery(P<0.01). CONCLUSIONS:Ulinastatin can inhibit the release of inflammatory factors TNF-α,IL-6 and IL-8 by inducing the expression of miR-155,which may be a new mechanism for the anti-inflammatory ef-fect of the drug in CPB cardiac surgery.
