Study on main components of water extract in Xiefei-Pingchuanling using UPLC-MS
10.3760/cma.j.issn.1673-4246.2017.06.013
- VernacularTitle:超高效液相色谱-质谱对泻肺平喘灵水提物的成分研究
- Author:
Jinhua WANG
;
Yuejia WANG
;
Jianguo CHAO
;
Juyan LIU
- Keywords:
Xiefei-Pingchualing;
Components of water extract;
Ultra performance liquid chromatography-tandem mass spectrometry
- From:
International Journal of Traditional Chinese Medicine
2017;39(6):530-534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS) chromatographic method for Xiefei-Pingchuanling extract, and to analyze the main components of its water extract. Methods The UPLC-MS was adopted with acetonitrile -0.2% formic acid aqueous solution (gradient elution) as mobile phase at a flow rate of 0.8 ml/min, detection wave length at 254 nm, and column temperature was 30 ℃. Results Eighteen components were separated from Xiefei-Pingchuanling extract, and eight ingredients were identified, such as the ephedrine, pseudoephedrine hydrochloride, salvianolic acidB, rhein, aloeemodin, chrysophanol, physcionwere. Conclusions The UPLC-MS chromatographic method has the specialty of stability and repeatability, and it is suitable for analysis of Xiefei-Pingchuanling extract.
