Role of Akt and SRC Pathways in Exosome?mediated Proliferation of Homologous Lung Adenocarninoma Cells

VernacularTitle:Akt和SRC在Exosomes促进同源肺癌细胞增殖中的作用
Author: Shilin XIE ; Jinglei QU ; Yibo FAN ; Xiaofang CHE ; Kezuo HOU ; Xiujuan QU ; Yunpeng LIU ; Xiaonan WANG ; Jian KANG ; Xuejun HU
Keywords: Exosomes; lung cancer; proliferation; Akt; SRC
From: Journal of China Medical University 2017;46(6):481-484
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of Exosomes isolated from the A549 lung cancer cells on the proliferation of these cells and their ho?mologous tumor cells,HCC827,and the role of the PI3K/Akt and SRC signaling pathways in this process. Methods Exosomes were isolated from the supernatant after density gradient centrifugation of A549 cells. The Exosomes morphology was observed by transmission electron microscopy. The expression of the Exosome?specific proteins was analyzed using Western blotting. Cell proliferation was investigated using the MTT assay. Re?sults The A549?derived Exosomes were 30?100 nm in diameter and had a bilayer membrane.Western blotting showed that CD9 was detected in these Exosomes. The isolated Exosomes promoted the proliferation of the A549 and the HCC827 cells in a dose?and time?dependent manner,ac?companied by the activation of Akt and SRC. Conclusion Exosomes isolated from A549 cells promote the proliferation of the secreting cells and the homologous tumor cells in a dose?and time?dependent manner. The mechanism may be related to the activation of Akt and SRC.