RNF180 Promoter Methylation in Prostate Cancer
10.12007/j.issn.0258?4646.2017.06.019
- VernacularTitle:RNF180启动子区甲基化在前列腺癌中的作用机制
- Author:
Haiguang WANG
;
Huamao JIANG
;
Zhirun ZUO
;
Huangzhe LONG
;
Guanyuan YUAN
;
Fanzhen JIA
- Keywords:
prostate cancer;
RNF180;
methylation of promoter
- From:
Journal of China Medical University
2017;46(6):561-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the significance of RNF180 expression in carcinogenesis and progression of prostate cancer by detection of RNF180 promoter methylation. Methods RNF180 expression was detected in human prostate cancer cell lines(PC3,LNCap,and DU145)and normal prostate cells(RWPE?1)via Western blotting,RT?PCR,methylation?specific PCR(MSP),bisulfite?sequeneing PCR(BSP),respectively, while RNF180 expression in human prostate cancer tissues and paired adjacent non?tumor tissue was detected via immunohistochemistry. Results The expressions of RNF180 mRNA and protein in prostate cancer cells were significantly lower than those in normal prostate cells(P<0.05),op?posite to what was observed for the methylation level of the RNF180 promoter. Additionally,the RNF180 expression in prostate cancer tissue was significantly lower than that in paired adjacent non?tumor tissue. Conclusion The RNF180 promoter is incompletely methylated in prostate can?cer cells,which may be a reason for the decline or silencing of RNF180 expression in cancer cells and tissues.
