Simultaneous determination of protocatechuic acid, salidroside, and chlorogenic acid in sargentodoxa cuneata by HPLC
10.3760/cma.j.issn.1673-4246.2017.06.014
- VernacularTitle:HPLC法同时测定大血藤中原儿茶酸、红景天苷和绿原酸含量
- Author:
Yuting HOU
;
Weijun GU
;
Bei XIANG
;
Huijie PAN
;
Yan SONG
;
Daming DENG
;
Ruxian DING
- Keywords:
Sargentodoxa cuneata;
Protocatechuic acid;
Rhodioloside;
Chlorogenic acid;
Chromatography,high pressure liquid
- From:
International Journal of Traditional Chinese Medicine
2017;39(6):535-538
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of protocatechuic acid, salidroside, and chlorogenic acid in Sargentodoxa cuneata. Methods The separation was performed on a Waters XSELECT CSH C18 (150 mm × 4.6 mm, 5 μm) with methanol-acetonitrile-0.2 % phosphoric acid as the mobile phase in a gradient elution at a flow rate of 0.8 ml/min. The detection wavelength was 260 nm and the column temperature was 35 ℃. Results The linear ranges of protocatechuic acid, salidroside, and chlorogenic acid were 0.0020-0.0120, 0.0600-0.3602, 0.0750-4.5006 mg/ml, respectively. The average recoveries were 98.01% (RSD=0.07%), 98.53 % (RSD=0.12%), and 101.10 % (RSD=1.92%), respectively. Conclusions The method is simple, accurate, and highly reproducible, which could provide the scientific evidence for the quality control of Sargentodoxa cuneata.
