The Effects of Valproic Acid on Macrophage Polarization Induced by Paraquat or Lipopolysaccharide
10.12007/j.issn.0258?4646.2017.06.016
- VernacularTitle:丙戊酸对百草枯与脂多糖诱导的巨噬细胞炎性极化的作用
- Author:
Renqing ZENG
;
Xizi WU
;
Yangzi ZHAO
;
Yunlei DENG
;
Shiyuan YU
;
Huiyi LI
;
Chang LIU
;
Chenling FAN
;
Hong WANG
;
Wei CHONG
- Keywords:
paraquat;
lipopolysaccharide;
valproic acid;
macrophage;
phenotypic polarization
- From:
Journal of China Medical University
2017;46(6):548-551,556
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects of valproic acid(VPA),a histone deacetylase(HDAC)inhibitor,on macrophage polarization in?duced by paraquat(PQ)or lipopolysaccharide(LPS). Methods Mouse RAW264.7 cells were cultured at 37℃with 5%CO2,passaged,and then given one of the following treatments:(1)PQ;(2)PQ+VPA(classⅠandⅡa HDAC inhibitor);(3)PQ+apicidin(classⅠHDAC inhibitor);(4)PQ+MC1568(classⅡa HDAC inhibitor);(5)LPS;(6)LPS+VPA;(7)LPS+apicidin;(8)LPS+MC1568. The cells and culture supernatants were harvested after 8 h of treatment. RT?PCR,ELISA,and flow cytometry were conducted to assess the expression levels of macrophage phenotyp?ic markers. Results Both PQ and LPS skewed the macrophage functional polarity toward proinflammatory phenotype. VPA,apicidin,and MC1568 all inhibited PQ?and LPS?induced macrophages polarizing toward pro?inflammatory phenotype ,but the inhibitory effects were different in some ways. Conclusion VPA inhibits the proinflammatory function of macrophages induced by PQ and LPS ,but the effect of VPA on PQ?and LPS?induced macrophages has its own characteristics.
