Correlation analysis between the imbalance of T cell subsets of the tissues and peripheral blood and ovarian cancer cell metastasis
10.3760/cma.j.issn.1673-4904.2017.05.002
- VernacularTitle:卵巢癌细胞转移与组织、外周血中T淋巴细胞亚群失衡的相关性分析
- Author:
Xiaoli LIU
;
Zhiming YANG
;
Lili WANG
;
Jing WANG
- Keywords:
Ovarian neoplasms;
T-lymphocyte subsets;
Neoplasm metastasis;
Immunity,cellular;
Retrospective studies
- From:
Chinese Journal of Postgraduates of Medicine
2017;40(5):390-394
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the imbalance of T cell subsets of the tissue and peripheral blood and ovarian cancer cell metastasis. Methods The surgical specimens of ovarian cancer of 89 patients who had underwent operation treatment were selected. Among them, local or distant metastasis was found in 40 cases (metastasis group), and no local or distant metastasis was found in 49 cases (non-metastasis group). The CD4 + and CD8 + T lymphocyte of ovarian cancer tissue, paraneoplastic tissue and peripheral blood were detected by flow cytometer, and the interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-αand interferon (IFN)-γof peripheral blood were detected by enzyme linked immunosorbent assay (ELISA). Results The CD4 + T lymphocyte of ovarian cancer tissue in metastasis group was significantly higher than that in non-metastasis group:0.25 ± 0.04 vs. 0.20 ± 0.03, the CD8+T lymphocyte of ovarian cancer tissue was significantly lower than that in non-metastasis group:0.21 ± 0.03 vs. 0.24 ± 0.04, and there was statistical difference (P<0.01). There were no statistical differences in CD4+and CD8+T lymphocyte of paraneoplastic tissue between 2 groups (P>0.05). The CD4+T lymphocyte of peripheral blood in metastasis group was significantly lower than that in non-metastasis group:0.25 ± 0.04 vs. 0.28 ± 0.04, and there was statistical difference (P<0.05). There was no statistical difference in CD8+T lymphocyte of peripheral blood between 2 groups (P>0.05). The IL-6 and TNF-αlevels of peripheral blood in metastasis group were significantly lower than those in non-metastasis group:(22.38 ± 5.58) ng/L vs. (25.82 ± 4.58) ng/L and (8.47 ± 2.35) ng/L vs. (10.74 ± 3.28) ng/L, the IL-10 level was significantly higher than that in non-metastasis group:(44.47 ± 12.26) ng/L vs. (39.48 ± 9.58) ng/L, and there were statistical differences (P<0.01 or<0.05). There was no statistical difference in IFN-γof peripheral blood between 2 groups (P>0.05). Conclusions The imbalance of T cell subsets in patients with ovarian cancer is mainly manifested by the drift of Th1/Th2 cell immunity to Th2 cell, which is related to the metastasis of the tumor.
