Role of TGF-β1 in multi-drug resistance in small cell lung cancer and its clinical significance
10.11817/j.issn.1672-7347.2017.04.009
- VernacularTitle:转化生长因子β1在调节小细胞肺癌耐药细胞对化学治疗药物耐药性中的作用及临床意义
- Author:
Huanxin LIU
;
Wei WANG
;
Guoxiang ZHANG
;
Linlang GUO
- Keywords:
transforming growth factor β1;
small cell lung cancer;
multi-drug resistance;
clinical prognosis
- From:
Journal of Central South University(Medical Sciences)
2017;42(4):419-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of transforming growth factor β1 (TGF-β1) in multi-drug resistance in small cell lung cancer and its clinical significance.Methods:The mRNA and protein expressions of TGF-β 1 in H69 and H69AR cells were detected by real-time PCR and Western blot,respectively.After silence of TGF-[β1,the sensitivity of H69AR to drugs was detected by CCK8 assay.The expressions of TGF-β1 in lung cancer and paracarcinoma tissues were examined by QRT-PCR and immunohistochemistry.The relationship of TGF-β 1 expression with clinical pathological features and prognosis of patients was studied.Results:Compared to H69,the mRNA and protein expressions of TGF-β1 in H69AR cells were significantly increased by (5.93±0.47) and (8.49±1.92) folds,respectively (P＜0.01).Transfection ofTGF-β1 siRNA resulted in a decrease of TGF-β1 expression by 70.432％ in H69AR ceils (F=21.20,P＜0.01) and an increase insensitivity to chemotherapeutic agents of H69AR cells (t=4.576,P＜0.05).Compare with the paracarcinoma tissues,the expression of TGF-β1 was significantly increased in small cell lung cancer tissues (t=13.925,P＜0.01),which was closely related with clinical stage,chemosensitivity and overall survival (all P＜0.05),but not related with gender,age (both P＞0.05).Conclusion:TGF-β1 is involved in the regulation of small cell lung cancer multidrug resistance,which may be a potential marker to evaluate the chemosensitivity and dinical prognostic for small cell lung cancer.
