Inhibitory effect of SM-1 on human liver microsomal cytochrome P450 enzyme
10.3969/j.issn.1001-1978.2017.05.008
- VernacularTitle:SM-1对人肝微粒体细胞色素P450亚型酶的抑制作用
- Author:
Yanfen CHEN
;
Jingxiu CHEN
;
Meng SUN
;
Junyao DING
;
Zeneng CHENG
;
Gaoyun HU
;
Jinsong DING
;
Qubo ZHU
- Keywords:
SM-1;
cytochrome P450;
substrate;
inhibition rate;
microsomes;
subtypes of enzymes
- From:
Chinese Pharmacological Bulletin
2017;33(5):627-629
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of SM-1 on seven main cytochrome P450(CYP450)in human liver microsomes.Methods Substrate or SM-1 was incubated with human liver microsomes for 30 min in vitro,and divided into control group and experimental group.The effects of SM-1 on the main phase I metabolic enzymes in human liver microsomes was detected by HPLC.Phenacetin,bupropion,paclitaxel,tolbutamide,omeprazole,dextromethorphan,testosterone were investigated as probe drugs.Results Inhibition rate of SM-1 on the classical substrate of human liver microsomal CYP was 0.05％,3.37％,0.08％,2.07％,4.20％,-0.15％and 10.84％,respectively.Conclusions SM-1 may have inhibitory effect on CYP3A4.Attention should be paid to the interaction of clinical drug induced by CYP enzyme inhibition.
