Effect of DPF2-RNAi on proliferation, apoptosis and cell cycle of PANC-1 cells
10.3969/j.issn.1001-1978.2017.05.012
- VernacularTitle:DPF2基因RNA干扰对人胰腺癌细胞PANC-1增殖、凋亡和细胞周期的作用研究
- Author:
Chao LIU
;
Ruyu SUN
;
Jian HUANG
;
Lihua LIU
;
Shengyun FANG
- Keywords:
DPF2;
RNAi;
lentivirus;
PANC-1;
proliferation;
apoptosis;
cell cycle
- From:
Chinese Pharmacological Bulletin
2017;33(5):647-652
- CountryChina
- Language:Chinese
-
Abstract:
Aim DPF2 has been reported to be involved in pathogenesis of leukaemia and oncogenic activity.However,the role of DPF2 in oncogenesis and pathogenesis of pancreatic cancer remains unclear.Therefore,the present research aims to investigate the effects of DPF2-RNAi on proliferation,apoptosis and cell cycle regulation of a pancreatic cell line,PANC-1 cells.Methods The lentivirus-mediated DPF2-RNAi was employed to knockdown DPF2 expression in PANC-1 cells,and the role of DPF2-RNAi in proliferation,apoptosis and cell cycle regulation of the PANC-1 cells was examined through MTT assay,colony formation assay and flowcytometry assay.Results The lentivirus-mediated DPF2-RNAi middle and high doses(2 μL and 4 μL)significantly decreased the expression of DPF2 in the PANC-1 cells.DPF2-RNAi decreased cell viability and colony formation,and increased apoptosis of the PANC-1 cells.Besides,DPF2-RNAi induced the S-phase arrest and decreased G2/M phase population of the PANC-1 cells.Conclusions DPF2 may play a crucial role in proliferation,apoptosis and cell cycle regulation of PANC-1 cells.Knockdown of DPF2 through lentivirus-mediated DPF2-RNAi may provide experimental basis for finding a new method for therapy of pancreatic cancer.
