Gene expression of human coronary artery endothelial cells in response to Porphyromonas endodontalis invasion.
10.5395/JKACD.2009.34.6.537
- Author:
Hee Joung KONG
1
;
Kyoung Kyu CHOI
;
Sang Hyuk PARK
;
Jin Yong LEE
;
Gi Woon CHOI
Author Information
1. Department of Conservative Dentistry, Division of Dentistry, Graduate of Kyung Hee University, Korea. gwchoi@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Porphyromonas endodontalis;
Atherosclerosis;
Cardiovascular disease (CVD);
Invasion;
Human coronary artery endothelial cells (HCAEC);
Gene expression
- MeSH:
Apoptosis;
Atherosclerosis;
Cardiovascular Diseases;
Coronary Vessels;
Cytokines;
Dental Plaque;
Dental Pulp Cavity;
Endothelial Cells;
Gene Expression;
Humans;
Microarray Analysis;
Myocytes, Smooth Muscle;
Oral Health;
Periodontal Pocket;
Periodontitis;
Porphyromonas;
Porphyromonas endodontalis;
Porphyromonas gingivalis;
Real-Time Polymerase Chain Reaction;
Sequence Homology;
Transcriptome
- From:Journal of Korean Academy of Conservative Dentistry
2009;34(6):537-550
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
During the last two decades, there has been an increasing interest in the impact of oral health on atherosclerosis and subsequent cardiovascular disease (CVD). To date, some periodontal pathogens including Porphyromonas gingivalis (P. gingivalis) have been reported to be relevant to CVD. Porphyromonas endodontalis (P. endodontalis), which shares approximately 87% sequence homology with P. gingivalis, is mostly found within infected root canals. However, recent studies reveal that this pathogen also resides in the dental plaque or periodontal pocket in patients with periodontitis. It has been shown that P. endodontalis invades human coronary artery endothelial cells (HCAEC) and coronary artery smooth muscle cells (CASMC). To evaluate whether P. endodontalis can participate in the progression of atherosclerosis and CVD, we examined the changes in transcriptional gene expression profiles of HCAEC responding to invasion by P. endodontalis in this study. The following results were obtained. 1. Porphyromonas endodontalis was invasive of HCAEC. 2. According to the microarray analysis, there were 625 genes upregulated more than two-folds, while there were 154 genes downregulated by half. 3. Upregulated genes were relevant to inflammatory cytokines, apoptosis, coagulation and immune response. Enhanced expression of MMP-1 was also noticeable. 4. The transcription profiles of the 10 selected genes examined by real-time PCR agreed well with those observed in the microarray analysis. Thus, these results show that P. endodontalis presents the potential to trigger and augment atherosclerosis leading to CVD.
