Influence of excessive PTEN expression to fibroblast cycle and collagen secretion induced by LPS
10.3969/j.issn.1673-4130.2017.09.012
- VernacularTitle:过表达PTEN对LPS诱导的成纤维细胞周期及胶原分泌的影响
- Author:
Yuekun ZOU
;
Zhiyuan SHI
;
Jing YI
;
Minhui ZHU
;
Ming ZHANG
;
Yaoyao SONG
;
Xiangbai YE
;
Yan YU
- Keywords:
LPS;
fibroblasts;
PTEN;
cell cycle;
collagen
- From:
International Journal of Laboratory Medicine
2017;38(9):1190-1191,1195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of excessive PTEN expression to fibroblast cycle and collagen secretion induced by LPS.Methods Normal skin fibroblast in the patient with hyperplastic scar were cultured in vitro.When the primary culture was close to 80% fusion,the digestive passage was performed,cultured to the third generation.LPS(0.5 μg/mL) was adopted to stimulate the third generation of normal skin fibroblasts.Defective adenovirus carrying PTEN gene was transfected to the third passage fibroblasts after LPS stimulation.Flow cytometer was adopted to detect the cell cycle.ELISA method was adopted to detect the secreted collagen amont.Results Excessive PTEN expression could inhibit the increase of G2M cell cycle induced by LPS.LPS stimulation could increase the secretion of collagen in skin fibroblasts,yet excessive PTEN expression could inhibit the secretion of collagen induced by LPS.Conclusion LPS could increase the amont of fibroblasts on G2M cell cycle and secretion of collagen,yet excessive PTEN expression can inhibit the effect.
