Analysis on results of four kinds of method in susceptibility testing of tigecycline against Acinetobacter baumannii
10.3969/j.issn.1673-4130.2017.09.015
- VernacularTitle:四种方法检测替加环素对鲍曼不动杆菌药敏结果分析
- Author:
Peihong YANG
;
Xiuli XU
;
Jiayun LIU
;
Yiqun SUN
- Keywords:
tigecycline;
Acinetobacter baumannii;
bacterial susceptibility testing
- From:
International Journal of Laboratory Medicine
2017;38(9):1198-1200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the sensitivity of four kinds of drug susceptibility test method in detecting sensitivity of tigecycline against Acinetobacter baumannii.Methods The susceptibility of 72 clinically isolated strains of carbapenemase-resistant Acinetobacter baumannii(CRAB) to tigecycline in vitro was detected with disk diffusion method,VITEK 2 Compact system,E-test and MIC test strip(MTS) test strip respectively,according to FDA standards,and the differences of four kinds of drug susceptibility test methods were compared.Results The susceptibility rates of 72 strains of CRAB to tigecycline by disk diffusion method,VITEK 2 Compact system,E-test and MIC test strip were 50.00%,69.44%,36.11% and 98.61% respectively,the intermediate rates were 48.61%,29.17%,26.39% and 1.39% respectively,the resistant rates were 1.39%,1.39%,37.50% and 0.00% respectively.Compared with MTS,the classification consistency rates of E-test,disk diffusion method and VITEK 2 Compact system were 36.11%,51.39% and 70.83% respectively.Conclusion There is difference among four kinds of method for conducting the drug susceptibility testing of tigecycline against CRAB,the consistency of disk diffusion method,VITEK 2 Compact system and E-test is lower.Detecting mediation or drug resistant strains of CRAB by disk diffusion method,VITEK 2 Compact system and E-test needs to be verified by MTS or Broth dilution method.
