Proteomic techniques based protein biomarkerssearching and validation in pleural effusion
10.19405/j.cnki.issn1000-1492.2017.05.017
- VernacularTitle:基于蛋白质组学的胸腔积液蛋白标志物筛选与验证
- Author:
Zhibin LI
;
Jun SHU
;
Jing MENG
- Keywords:
immunoglobulin λ;
haptoglobin;
proteomics;
pleural effusion;
diagnosis
- From:
Acta Universitatis Medicinalis Anhui
2017;52(5):700-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through analyzing benign and malignant pleural effusion samples by proteomic techniques, finding protein biomarkers to provide help and new clues for effusion differential diagnosis.Methods Two-dimensional electrophoresis(2-DE) and matrix-assisted laser desorption ionization-time of flight-mass spectrometry(MALDI-TOF-MS) were used to search and identify protein biomarkers, enzyme linked immunosorbent assay(ELISA) was to validate the biomarkers.Results By comparing malignant group with benign group, 43 significantly different protein spots(Up or down regulated≥2 times) were found.Including 9 up regulated spots and 34 down regulated spots.And 7 spots were identified(Up or down regulated≥3 times) by MALDI-TOF-MS and validated 2 spots immunoglobulin λ(Igλ) and haptoglobin(Hp) by ELISA.The results showed that Igλ showed no statistical significance between two groups, while Hp showed the statistical significance(P<0.05).The diagnostic sensitivity and specificity of Hp in malignant pleural effusion were 75.00% and 52.38% at diagnostic cut-off point of 389.02 μg/L.Conclusion The application of proteomics technology has a great help with protein biomarkers searching in pleural effusion.HP has a certain value for the differential diagnosis of benign and malignant pleural effusionand and worthy of further study.
