Influence of different storage ways on the testing results of AD7C-NTP in urine specimen
10.3969/j.issn.1673-4130.2017.11.003
- VernacularTitle:不同保存处理对尿标本AD7C-NTP检测结果的影响研究
- Author:
Chengjie XU
;
Zhibin WANG
;
Jie ZHANG
;
Yibin TANG
;
Taichang TAN
- Keywords:
neuronal thread protein;
Alzheimer's disease;
storage;
urine
- From:
International Journal of Laboratory Medicine
2017;38(11):1447-1448,1451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of different preservation conditions and time period on the testing results of Alzheimer's disease(AD) associated neuronal thread protein(AD7C-NTP) in urine specimen.Methods From Oct.2015 to Jan.2016,urine specimen were collected from 50 AD patients,and divided into three groups,according to the different storage temperature,including room temperature group,4 ℃ group and-20 ℃ group.Preservatives were added into specimen of 4 ℃ preservation group and-20 ℃ preservation group.AD7C-NTP level was detected at different preservation time of all specimen.Results The testing results of AD7C-NTP in specimen of room temperature group and 4 ℃ groups,detected within three days,were not significantly different with initial detection value(P>0.05).After seven days,the testing results in specimen of 4 ℃ group were not significantly different with initial detection value(P>0.05).However,after one day,the testing results in specimen of-20 ℃ group were significantly different with initial detection value(P<0.05).Contrast with 4 ℃ without preservative group,the adding of preservative could not increase the stability of AD7C-NTP.The adding of preservative in specimen of-20 ℃ could obviously increase stability,but the deviation of testing results was beyond acceptable limits.Conclusion 4 ℃ without preservatives could be the optimal storage condition for detection of AD7C-NTP in the urine.
