Effect of microRNA-155 on sorafenib resistance in hepatocellular carcinoma

VernacularTitle:MicroRNA-155在肝细胞癌对索拉非尼抗药中的作用研究
Author: Feng LYU ; Wei WANG ; Xin JIN ; Dewei LI
Keywords: mircoRNA-155; hepatocellular carcinoma; sorafenib; drug resistance; lentivirus; activated caspase-3网络出版时间:2017-4-24 11:20
From: Chinese Pharmacological Bulletin 2017;33(5):657-662
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of microRNA-155(miR-155)on sorafenib resistance in hepatocellular carcinoma(HCC).Methods Lentivirus mediated miR-155 inhibition was transfected into SMMC-7721 cells,while lentivirus mediated miR-155 overexpression was transfected into HepG2 cells.The level of miR-155 was evaluated by qPCR.Cell viability and apoptosis were analyzed by cell counting kit-8(CCK-8)assay and flow cytometry,respectively.The protein expression of activated caspase-3 was measured by Western blot.Results Compared to control group,the expression of miR-155 was significantly downregulated in miR-155 inhibition lentivirus infected SMMC-7721 cells(P<0.01),sorafenib treatment markedly suppressed cell viability(P<0.05)and increased cell apoptosis(P<0.01),as well as enhanced the expression of activated caspase-3(P<0.01).However,HepG2 cells were infected by miR-155 overexpression lentivirus which deserved completely opposite results.Conclusion miR-155 may participate in sorafenib resistance in HCC and provide a promising molecular target for the treatment of HCC.