Based on the cecum ligation perforation animal models of sepsis in the rat lung inflammation and oxidative stress observation
10.3969/j.issn.1005-1678.2017.09.005
- VernacularTitle:基于盲肠结扎穿孔脓毒症大鼠动物模型的肺部炎症及氧化应激观察
- Author:
Lijuan CAI
;
Yan ZHANG
;
Xuejun DING
;
Zongwei CHEN
- Keywords:
sepsis;
rat animals;
oxidative stress
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(9):20-21,25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the pulmonary inflammation and oxidative stress in rats with cecal ligation and puncture sepsis. Methods The male rats in 8 weeks were randomly divided into control group, sham operation group and model group,The ALI model of sepsis was prepared by cecal ligation and puncture (CLP). In order to achieve the effect of experimental observation, the rats were killed after the operation 12 h, and the lung tissue HE staining analysis was performed, and the levels of MPO and MDA were observed. Results The degree of lung injury and the levels of MPO and MDA in the model group were significantly higher than those in the control group and the sham operation group (P < 0.01).Conclusion Rats with cecal ligation and puncture sepsis model can well simulate sepsis inflammatory response and oxidative stress.
