Study on the Determination of β - Eucalyptol in Magnolia officinalis by Headspace Gas Chromatography
10.3969/j.issn.1005-1678.2017.09.008
- VernacularTitle:顶空气相色谱法测定厚朴中β-桉油醇含量的方法研究
- Author:
Chaqing WU
;
Faying WANG
;
Xiaojuan GUAN
- Keywords:
headspace gas chromatography;
β-eucalyptol alcohol;
magnolia
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(9):29-30,33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of headspace gas chromatography for the determination of β-eugenol in Magnolia officinalis. Methods Headspace gas chromatography was used. The column was HP-INNOWax capillary column, the detector was hydrogen flame ionization detector, inlet temperature: 200 ℃; column temperature: 135 ℃ for 3min, at 10 ℃/min heating rate rose to 150 ℃, At a rate of 15 ℃/min up to 250 ℃for 5 min; constant flow rate: 0.8 mL/min; detector temperature: 250 ℃; headspace injection, gas injection volume: 1 mL, headspace Temperature 180 ℃, the balance time 30min; with the external standard method according to the peak area calculation results. Results The linear range of the concentration of menthol was 2.49～24.86 μg/mL (r=0.9994). The RSD of the precision, stability and repeatability test were <5％, the average recovery was 97.0％, RSD was 0.84％ (n=9). Conclusion The method is simple and accurate for the determination of volatile oil.
