Effects of siRNA silencing EZH2 gene on invasion andmigration of human cervical cancer cell lines
10.7652/jdyxb201705012
- VernacularTitle:siRNA沉默EZH2基因对人宫颈癌细胞侵袭转移的影响
- Author:
Jing JI
;
Kangrong HUANG
;
Huifang HUANG
;
Yueling WANG
;
Jing FANG
- Keywords:
enhancer of zeste homolo 2 (EZH2) gene;
cervical cancer;
siRNA;
invasion and migration
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(5):684-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of siRNA silencing enhancer of zeste homolo 2 (EZH2) on invasion and migration of cervical cancer cells and the molecular mechanisms.Methods The small interfering RNAs (siRNA) targeting EZH2 were transiently transfected into C33A cell line by lipofectamin2000.The effects of EZH2 on cell invasion and migration were detected by wound-healing assay, Transwell assay and soft agar colony assay.The expression of MMP2 was detected by Western blot.Results Downregulation of EZH2 expression by siRNA in C33A cell line significantly inhibited cell invasion and migration in vitro.Meanwhile, siRNA-mediated depletion of EZH2 reduced the expression of MMP2.Conclusion Knocking down EZH2 expression by siRNA could surpress invasion and migration of human cervical cancer cells, which might be related to downregulating MMP2 expression.
