Inhibition Effect of Zoledronic Acid on Metastasis of Esophagus Squamous Cell Carcinoma

VernacularTitle:唑来膦酸对食管鳞癌细胞转移的抑制作用
Author: Shubo XIN ; Yanjie YOU ; Canfeng LIN
Keywords: Zoledronic acid; Esophagus squamous cell carcinoma; Cell proliferation; Neoplasm invasiveness; Occludin
From: Herald of Medicine 2017;36(8):869-874
CountryChina
Language:Chinese
Abstract: Objective To examine the inhibition effect of zoledronic acid (ZOL) on malignant metastasis of human esophagus squamous cell carcinoma (ESCC) cells and to analyze its molecular mechanisms.Methods EC9706 and EC109 cells were treated with ZOL,and then MTT assay,adhesion and invasion assay were performed to observe the inhibitory effect of As2O3 on proliferation and metastasis of esophagus carcinoma cells.The expression of metastasis-related proteins was detected by Western blotting.Results Exposure to ZOL significantly presented suppressive functions on growth and metastasis of both kinds of cancer cells,in a dose-dependent manner(P＜ 0.05).Additionally,the expression level of occludin was increased after ZOL treatment by suppressing transcriptional factor Slug.Transfection of Slug could reverse anti-metastasis of ZOL.Conclusion ZOL possesses a significant anti-metastasis function on ESCC cells,mainly through repressing Slug to restore occludin expression.