Determination of triterpenes in Melastoma dodecandrum Lour. from different producing areas by HPLC
10.3760/cma.j.issn.1673-4246.2017.09.014
- VernacularTitle:HPLC法测定不同产地地菍中三萜类成分含量
- Author:
Bo ZHANG
;
Qinwen ZOU
;
Ruichao LIN
- Keywords:
Chromatography;
high pressure liquid;
Melastoma dodecandrum Lour.;
Asiatic acid;
Betulinic acid;
Oleanolic acid
- From:
International Journal of Traditional Chinese Medicine
2017;39(9):824-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.
