Solanine induces the apoptosis of human prostate cancer cells via ROS/p38 signaling pathway

VernacularTitle:龙葵素通过ROS/p38信号通路诱导人前列腺癌细胞凋亡
Author: Weifeng ZHONG ; Nanhui CHEN ; Yuqing HUANG ; Pei WAN ; Yifeng LIN ; Huiming JIANG ; Kaihua ZHONG ; Bin PAN ; Siping LIU
Keywords: solanine; apoptosis; prostate cancer; p38
From: Journal of Xi'an Jiaotong University(Medical Sciences) 2017;38(3):457-461,封3
CountryChina
Language:Chinese
Abstract: Objective To investigate the molecular mechanism of solanine-induced apoptosis of prostate cancer cells Du145 and LNCaP.Methods The effects of solanine on the viability of Du145 and LNCaP cells were evaluated by MTT assay.The generation of intracellular reactive oxygen species (ROS) and solanine-induced apoptosis were measured by flow cytometry.The protein levels of p38 and p-p38 expressions were examined by Western blot.Results Solanine significantly inhibited the viability of Du145 and LNCaP cells in a dose-dependent manner (P＜0.01).The inhibition of solanine on cell viability was suppressed by the ROS scavenger NAC.ROS generation,apoptosis and phosphorylation of p38 were induced by treatment with solanine at 40 μmol/L for 24 h.The expression of p38 and solanine-induced apoptosis were suppressed by NAC and SB203580.Conclusion Solanine induces the apoptosis of human prostate cancer cell via the RO.S-p38 signaling pathway.