Protective effect of Sika deer velvet antler protein on cisplatin-induced kidney injury
10.3867/j.issn.1000-3002.2017.06.010
- VernacularTitle:梅花鹿鹿茸总蛋白对顺铂诱导肾损伤小鼠的保护作用及其机制
- Author:
Huihai YANG
;
Lulu WANG
;
Xiaofeng HE
;
Hang SUN
;
Fangfang LIU
;
Jing ZHANG
- Keywords:
Sika deer antler protein;
cisplatin;
nephrotoxicity;
renal protection
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(6):561-567
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the protective effect of Sika deer velvet antler protein (SVPr) against renal toxicity in mice and its mechanism.METHODS Forty ICR mice were randomly divided into 5 groups:normal control group (ig distilled water),model group (ig distilled water for 7 d,on the 7th day,ip cisplatin 25 mg·kg-1 to establish the model,afterwards ig distilled water for 3 d) and SVPr 5,10 and 20 mg· kg-1 groups (ig SVPr for 7 d,cisplatin 25 mg· kg-1 was provided 2 h after the last administration,then ig SVPr for 3 d).Testing kits were adopted for the measurement of renal indexes in mice,such as blood urea nitrogen (BUN) and serum creatinine (SCr);oxidative stress indictors of super oxide dismutase (SOD),catalase (CAT),glutathione (GSH) and malondialdehyde (MDA);inflammation indictor levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).Caspase 3,Bax and Bcl-2 were detected via Western blotting,and renal pathological changes were observed by HE staining.RESULTS SVPr (5,10 and 20 mg·kg-1) significantly reduced the levels of SCr,BUN,MDA,TNF-α and IL-6,and the expressions of caspase 3 and Bax (P＜0.05),but increased the activities of SOD,CAT and GSH,and the expression of Bcl-2 (P＜0.05).The renal pathological changes were improved.CONCLUSION SVPr can reduce renal toxicity induced by cisplatin in mice,and the mechanism is probably related to inhibiting oxidative stress or inflammatory reaction and improving cell apoptosis.
