Identification of Bletilla Striata (Thunb.) Reichb.f.and Bletilla Formosana (Hayata) Schltr.Based on ITS2 Sequence
10.11842/wst.2017.05.022
- VernacularTitle:基于ITS2序列鉴定白及与其伪品小白及
- Author:
Ying LUO
;
Zhili ZHAO
;
Keli CHEN
;
Yimei LIU
- Keywords:
Bletilla striata;
Bletilla formosana;
ITS2 sequence;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2017;19(5):841-845
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to identify Bletilla Striata (Thunb.) Reichb.f.and Bletilla Formosana (Hayata) Schltr.by ITS2 sequence.The leaves of 38 samples of Bletilla striata and Bletillaformosana from Yunnan,Hubei,Guizhou,Hunan and Sichuan province were used as experiment materials.The total DNA was extracted.Internal transcribed spacer 2 (ITS2) sequences were obtained by PCR.All of the ITS2 sequences were checked.The 8 ITS2 sequences from two species were downloaded from GenBank.The intraspecific and interspecific Kimura-2-parameter (K2P) distances of Bletilla striata and Bletilla formosana were calculated by MEGAS.0.And neighbor-joining (NJ) tree was constructed.The results showed that the full-length sequences of ITS2 from Bletilla striata and Bletillaformosana were 259 bp,with a total of 14 variable sites.The maximum intraspecific K2P distance of Bletilla striata and Bletillaformosana was 0.008,while the minimum interspecific K2P distance was 0.040.The ITS2 secondary structure showed that different origins of Bletilla striata were gathered together and could be distinguished obviously from Bletilla formosana by NJ tree.It was concluded that ITS2 sequence was able to identify Bletilla striata and Bletillaformosana quickly and accurately.
