Expression of TLR4 signal pathway and pIgR in SE-induced inflammation response
10.16303/j.cnki.1005-4545.2017.08.20
- VernacularTitle:SE感染对雏鸡空回肠组织中TLR4信号转导通路及pIgR的影响
- Author:
Dejun LI
;
Xiaoling ZHOU
;
Yunfeng LIU
;
Xue SONG
- Keywords:
SE;
TLR4;
jejunum;
ileum;
pIgR
- From:
Chinese Journal of Veterinary Science
2017;37(8):1545-1548
- CountryChina
- Language:Chinese
-
Abstract:
To observe the effect of pIgR on Salmonella Enteritidis induced inflammation in jejunum and ileum in Chicken,7 day Hyline chickens were taken orally with Salmonella Enteritidis (SE) and killed after 1 d,3 d,7 d and 14 d.The mRNA expression of Toll-like receptor 4(TLR4),MyD88,TRAF6,NF-κB and pIgR was detected by real-time RT-PCR and pIgR protein level was detected by Western blot.The results showed that TLR4 signaling pathway was activated and mR-NA level of the pIgR in jejunum and ileum was enhanced (P＜0.01) and protein level of the pIgR in jejunum and ileum was up-regulated by SE.The study proved that TLR4 signal pathway on mucosal cell surface of jejunum and ileum was activated and expression of pIgR was up-regulated and gut mucosal immunity of chicken was strenghtened.
